Pcold f
SpletpCold III. 编号 北京华越洋生物VECT4570 载体名称 pCold III pColdIII载体基本信息 载体名称: pCold III, pColdIII, pCold-‐III 质粒类型: 大肠杆菌蛋白表达载体 表达水平: 高 启动子: CSPA 克隆方法: 多克隆位点，限制性内切酶 载体大小: 4377 bp 5' 测序引物及序列: pCold-‐F: 5'-‐ACGCCATATCGCCGAAAGG-‐3' 3' 测序引物及 ... SpletT N ( N Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. pCold-F 19-mer / 58% GC …
Pcold f
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SpletpCold™ ProS2 DNA: 25 ug: USD $798.00: Takara's pCold ProS2 DNA is a cold shock expression vector that includes Protein S derived from a Gram-negative myxobacteria … SpletpCold™ TF DNA - タカラバイオ株式会社 遺伝子工学研究. 製品コード 3365 研究用 pCold™ TF DNA 説明書 v201411Da タンパク質の構造や機能の解明はポストゲノムの重要な研究対象で、効率のよいタンパク質生産システ ムはポストゲノム解析に必須の基盤技術です ...
Splet02. sep. 2024 · pCold TF DNA 的研发则避免了转化操作的复杂性，它是一种以大肠杆菌的伴侣蛋白Trigger Factor (TF)作 为可溶性标签的融合型冷休克表达载体。. TF 分子量为48 kDa ，Trigger Factor 是一种原核的核糖体结合伴 侣蛋白质，能够促进新生肽链的共翻译折叠。. TF 来源于大肠杆菌 ... Splet提供最终幻想9(Final Fantasy 9)(FF9)辅助技能一览word文档在线阅读与免费下载，摘要:※该表按技能英文名首字母顺序排列。※MP为装备该技能需要的魔石数。※人物为可以习得该项辅助技能的人物，其中ALL为全8人都可习得，Z为吉坦，D为嘉妮特(小刀)，V为比比，S为斯坦纳，F为芙蕾娅，E为艾可，A为撒拉 ...
SpletpCold-F 19-mer / 58% GC 1 binding site 208 .. 226 = 19 annealed bases T m = 60°C pCold-R 20-mer / 50% GC 1 binding site 384 .. 403 = 20 annealed bases T m = 56°C lacI 3162 .. … http://www.biofeng.com/zaiti/dachang/pColdTF.html
SpletpCold TF载体说明. pColdTF 载体简介 pCold TF 载体是一种融合表达可溶性的标签"触发因子 （Trigger Factor,TF） 伴侣"的冷休克载体。. "触发因子"（48KD）是原核核糖体相关的分子伴侣蛋白（48 kDa） ，有利于新表达的多肽的共翻 译折叠。. 正因为它来源于大肠杆菌，保证 …
Splet17. avg. 2024 · Hence, the pCold-TF-BpFae expression vector was constructed, and the target fusion protein TF-BpFae was overexpressed at 15 °C in E. coli BL21 (DE3) cells. TF-BpFae has a predicted MW of 111 kDa . The results of SDS-PAGE analysis from total protein and supernatant fractions showed that, in the presence of TF and the cold-shock … story hustler bookSplet浩格生物科技有限公司是专业的pcambia1300-35S-EGFP-VPE1HH-ZW-057厂家,为您提供价格优惠最佳解决方案,我们有pcambia1300-35S-EGFP-VPE1HH-ZW-057各种型号、资料、使用说明PDF文档,采购pcambia1300-35S-EGFP-VPE1HH-ZW-057尽在浩格生物科技有限公司 story ia assessorSpletTakara Bio's pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed polypeptides. Because of its E. coli origin, TF is highly story hut youtubehttp://www.app17.com/c145959/products/d11302692.html ross service north tonawandaSpletC1801 pCold-SUMO protein kit. 重组质粒构建 1.根据目的基因选择合适的酶切位点,将 pCold-SUMO 载体和插入片段分别进行双酶切,按如下反应进行双酶切反应(插入 目的基因片段与载体相同) pCold-... C1801 pCold-SUMO protein kit. C1801 pCold-SUMO protein kit_生物学_自然科学_专业资料。 ross setzler real estate brownwood texasSpletPlasmid pCold-TF-hRubicon from Dr. Tamotsu Yoshimori's lab contains the insert Rubicon and is published in Mol Biol Cell. 2010 Dec;21(23):4162-72. doi: 10.1091/mbc.E10-06-0495. Epub 2010 Oct 13. This plasmid is available through Addgene. × Close Image for pCold-TF-hRubicon. Generated by Addgene from plasmid data. ... rosss for suc dbmSplet11. avg. 2024 · Active BpEG01790 was successfully overexpressed with or without the signal peptide using the pCold TF vector expression system and E. coli BL21 (DE3) cells. Overexpression of recombinant BpEG01790 without the signal peptide was higher compared with the construct that included the signal peptide. story-i